Brain spatial signal acquisition and real time analysis system

Neurobot’s multi-channel data acquisition and analysis system is one of the most advanced electrophysiological recording, synchronous experimental control and data analysis systems, which can record the population neurons in animal behavior experiments. Its powerful compatibility makes it possible to conduct experiments simultaneously with other neurobehavioral instruments. The biological signal recording and data processing system specially designed for life science has replaced the traditional multi-channel physiological recorder, oscilloscope, X-Y recorder, stimulator and other instruments. It can be applied to the biological experiments of physiology, pharmacology, pathophysiology, exercise physiology and psychology in Colleges and universities. It can run under Windows 2000 / XP, The whole system consists of hardware system and software system. It is a multi-channel data acquisition system for animal neurophysiology experiment, with powerful function and easy operation. The system is an advanced multi-channel system with up to 1024 electrodes, which can record and analyze the electrical activities of animal brain and peripheral nerves. The system configuration can record the electrical signals in the body of animals (birds, mice, rats, cats, primates) under anesthesia or awake state. It also supports the recording of microelectrode plates of cultured cells and chambers of brain slices. Preamplifier and neural signal processor can collect, process and analyze real-time action potentials (spikes), field potentials, other physiological signals and experimental state. The preamplifier is compatible with hard single electrode, microelectrode and microelectrode array, planar silicon electrode, subdual ECoG grids, and epidural and scalp EEG electrodes. Flexible I / O options make it easy to connect third-party devices such as behavior, stimulation, and video systems.

The system has convenient user experience, easy to learn operation interface, professional software and strong compatibility. It can be used in experiments with other neurobehavioral instruments simultaneously.

characteristic:

It can support 16 channels, 32 channels, 48 channels, 80 channels, 112 channels, 256 channels, 512 channels and 1024 channels according to the number of connected modules (different modules can form integration unit). Low frequency signals such as EEG can be recorded in all channels.

Multi channel data acquisition system can be used for computers with USB 2.0 / USB 3.0 interface.

Ultra high sampling rate, sampling rate: 16K to 68KHz adjustable, 24bit.

Software selection, signal line selection, reference channel setting and control record are very simple and clear, flexible reference selection, 16 references can be selected for every 32 channels, which can be selected in software.

The software can automatically and manually classify the action potential clusters on the four electrodes, automatically calibrate and match the action potential and motion trajectory, real-time analyze multiple spatial position signals such as position, grid, head direction, boundary, velocity and joint cell, and realize multiple functions such as velocity filtering, spatial smoothing of discharge frequency map and action potential feature extraction.

During the recording process, as long as the discharge recorded by any channel can trigger multiple electrodes of the same group at the same time.

Good system compatibility: win, MAC, Linux

Basic parameters:

The types of recorded data include original wide band signal, high frequency spike signal and low frequency field potential (LFP).

It supports single eletrode, stereotrode and tetrode recording, and the recording mode includes unit recording mode and original data mode. The data can be imported into Matlab for analysis and output in text format The controlled parameters of digital oscilloscope include low cut filter: 0.05-4hz, high cut filter: 8khz-6th order Bessel, maximum magnification 3000x, positioning and triggering, etc. The display parameters include time parameter of scanning speed, trigger mode and grouping, signal and electrode channel, magnification, filtering and signal recording mode. Each channel can set digital filter independently.

More than 16 channels can be displayed at the same time, and the scanning rate of each channel is 0.1ms. The peak value and average value can be detected under low-frequency scanning frequency. The data of discharge, head direction, velocity and position, field potential and other low-frequency analog signal data can be displayed in real time during recording. The beginning and end of recording can be controlled by digital signal input.

Analog signal input and output, auxiliary acquisition or selection of headstage channel output (a single headstage can record multiple free moving animals at the same time, etc.)

18 24bit TTL digital inputs and outputs. The digital I / O module can integrate the discharge record with other kinds of experimental data.

Provide LTP data analysis module to analyze the slope, amplitude and width of EEG waveform. It can automatically analyze the firing characteristics of hippocampal neurons and synchronously correlate firing frequency with various behaviors. The recorded parameters of the analysis software include peak amplitude, initial latency and amplitude at specific position of the waveform.

Recording bandwidth: DC to 16KHz

System integrated stimulator, amplitude: 0-100V, step size: 1mV; Output current: 100mA; Wave width: 0.1-600ms, program-controlled step size: 0.1ms; Time delay: 1-250ms, programmable adjustment step 1ms.

Micro thruster: stainless steel 45 ° Tilt type, support to carry more than 32 electrodes. Step 24 μ m。 The head stage was lengthened; Platinum iridium wire, 17um (0.0067); Tungsten wire, 125um (0.0005)

Common mode rejection ratio > 160db

Equipped with 128 channel electrode electroplating device, the measurement range can reach 10K Ω- 500M Ω， The measurement accuracy can reach ± 0.1%（5k Ω- 10M Ω）； Frequency range: 1Hz ~ 10kHz, test current < 0.9na RMS; Electroplating range: ± fifteen μ A， ± 5V, electroplating resolution: 10na, 20mV; USB connection.

It includes at least three ways to detect and record signals. The instantaneous signal can be captured by screenshot, and the previous collected data and video files can also be played back at low speed or saved in sections.

It includes a video tracker (integrated and external), which can record the discharge signal and simultaneously record the animal’s position in real time, and record the discharge data and position data synchronously. Video tracker operation mode: big / small point mode (need two different LED lights), or single point mode (fixed position), two modes can be selected at will. It can be independent or integrated on the controller.

Online impedance test: electrode in vivo impedance test function

Large input range ensures no signal overflow: ± 10 mV

After the discharge signal is separated, the discharge time and the position of the animal at that time can be superimposed on the position map, the display results can be fitted, and the contour display can also calculate the position and the highest discharge frequency in a certain position.