Automatic patch-clamp system

Main features:

The automatic control system can not only detect the surrounding cells automatically, but also adjust the pressure control micro thruster to automatically detect the increase or decrease of electrode resistance of cell electrode, automatically detect neurons and form high resistance sealing. G ohm sealing, membrane breaking and recording software automatically calculate and automatically control without human intervention.

When the electrode enters the experiment, the system can control and automatically calculate the positive pressure output accurately. After the cell is formed with high resistance sealing, the system can automatically calculate and accurately control the negative pressure output.

The system can sequence single cells and experiment rats under awake and anaesthesia.

The system can also be used in the experiment of tissue slice in vitro. The software control is simple, convenient and flexible, and has a wide scalability.

Operation:

The control system can be used with the software developed by our company. It will issue appropriate serial commands to the control unit during operation to obtain the whole cell record. These commands are listed here for reference or part 3 development.

The software also controls the motor position connected to the probe, which works in collaboration with Newport pzc200 KT.

The computer control pressure system in the control unit can switch between the patch clamp and the four discrete pressures of each stage of the following serial command:

Pressure range (mbar) command

Atmosphere 0 switchatom

Low pressure 15-344 switchlow

High pressure 50-1000 switchhighp

Low 7.5-150 switchlow

7.5-150 switch at high altitude

All pressures can also be changed by knob in unit or using software serial port command panel.

USB cable to PC

COM port will appear in Windows Device Manager to connect to the computer via USB.

Effective value of pressure command

Low pressure setlowp, 15-344

High pressure sethighp,  50-1000

Low altitude setlowv, 7.5-150

High altitude sethighv, 7.5-150

The representative of #, is ordered to accept the number

For example, set pressure command:

setlowP,025

The minimum output negative pressure of the above command is 25mbar

setlowP,025

technical specifications

Maximum input pressure 150psi

Minimum input pressure 50psi

Power supply requirement 24 vdc/3a

High pressure output range 0-20psi

Low pressure output range 0-5psi

Low / high vacuum output range 0-14psi

Transmission speed USB 2.0

Compatible operating system win7

The control system also has breakthrough command, which can apply high negative pressure to electrode (MS) according to the interval time specified by the user

For example, set the break through command:

breakIn,300

“Breakin” is the instruction, 300 is the length of time, in milliseconds, and high negative pressure will act on the electrode.

There is also an embedded relay control unit, switch, analog output for the internal command connection of patch clamp amplifier, and the internal data and external input signals between the analog output of the switch are connected to the analog BNC connected to the front panel. Here is the command to switch the relay.

Sample relay switch command:

BNCswitch,ON

The two valid parameters of this command are on or off

These are general settings for serial communication

Serial port setting

Baud rate 9600

Data bit 8

Parity either side

Stop bit 1